Bovine adenovirus type 3 virions cannot be rescued in vivo after full-length viral genome transfection in the absence of detectable polypeptide IX.
10.4142/jvs.2017.18.2.217
- Author:
Peng ZHANG
1
;
Qinghong XUE
;
Jing MA
;
Jingjing REN
;
Shuili XIA
;
Lu ZHANG
;
Wenbin WANG
;
Suresh K TIKOO
;
Enqi DU
Author Information
1. College of Veterinary Medicine, North-west A&F University, Yangling 712100, China. duenqi227@126.com
- Publication Type:Original Article
- Keywords:
adenovirus;
bovine adenovirus type 3;
polypeptide IX;
viral rescue
- MeSH:
Adenoviridae*;
Adenoviruses, Human;
Biology;
Codon, Initiator;
Genetic Therapy;
Genome;
Genome, Viral*;
Leucine Zippers;
Species Specificity;
Transfection*;
Virion*
- From:Journal of Veterinary Science
2017;18(2):217-227
- CountryRepublic of Korea
- Language:English
-
Abstract:
Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative leucine zipper element (PLZP) were essential for virion rescue, whereas the C-terminus following the coiled-coil domain was non-essential. In addition, swap of the PLZP element and its following region of BAdV3 pIX to corresponding domains of human adenovirus type 5 (HAdV5) did not affect virion production, whereas swap of the entire pIX abolished production of progeny virions. We suggest that failure of the full-length BAdV3 pIX swap might be due to species specificity of its N-terminus region before the PLZP element.
