Cloning and expression of a human liver cancer related gene
10.3760/j:issn:0529-5815.2001.03.005
- VernacularTitle:一个新的人类肝癌相关基因的克隆和表达
- Author:
Zhengxu WANG
1
;
Hongyang WANG
;
Mengchao WU
Author Information
1. Eastern Hospital of Hepatobiliary Surgery Second Military Medical University
- From:
Chinese Journal of Surgery
2001;39(3):185-187
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To study and clone a novel liver cancer related genes, and to explore the molecular basis of liver cancer genesis. Methods Using mRNA differential display polymerase chain reaction (DDPCR), we investigated the difference of mRNA in human hepatocellular carcinoma (HCC) and paired paracarcinoma tissue, and got a gene probe. By screening the placenta cDNA library and genomic homologous extend, we got a full-length cDNA named STW-2. We analyzed the expression of this novel gene in 42 pairs of human hepatocellular carcinoma, paracarcinoma and normal tissue by means of Northern Blot. Results We have got a full-length cDNA of liver cancer associated gene STW-2 submitted to GeneBank nucleotide sequence databases (Accession No. AF276707), which localized at chromosome 18p11.2. The positive expression rate of this gene was 78.6% (33/42) in hepatocellular carcinoma tissue, and the clinical pathological data showed that the STW-2 was closely related to the completion of tumor envelope and adjacant small satellite nodules lesions (P＜0.05). The STW-2 was widely distributed in human normal tissue that was highly expressed in human lunge, brain tissue, and lowerly expressed in liver tissue. Conclusion A novel full-length cDNA differentially expressed was obtained in liver cancer, which was closely related to the invasive and metastatic tumor, and may be the later heredited change in HCC genesis.
