Antibiotic resistance patterns and genetic relatedness of Enterococcus faecalis and Enterococcus faecium isolated from military working dogs in Korea.
10.4142/jvs.2017.18.2.229
- Author:
Kiman BANG
1
;
Jae Uk AN
;
Woohyun KIM
;
Hee Jin DONG
;
Junhyung KIM
;
Seongbeom CHO
Author Information
1. BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea. chose@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Enterococcus faecalis;
Enterococcus faecium;
antibiotic resistance;
large-breed dog;
pulsed-field gel electrophoresis
- MeSH:
Adolescent;
Adult;
Animals;
Anti-Bacterial Agents;
Ciprofloxacin;
Dogs*;
Drug Resistance, Microbial*;
Electrophoresis, Gel, Pulsed-Field;
Enterococcus faecalis*;
Enterococcus faecium*;
Enterococcus*;
Gastrointestinal Tract;
Gentamicins;
Humans;
Imipenem;
Kanamycin;
Korea*;
Military Personnel*;
Multiplex Polymerase Chain Reaction;
Opportunistic Infections;
Streptomycin
- From:Journal of Veterinary Science
2017;18(2):229-236
- CountryRepublic of Korea
- Language:English
-
Abstract:
Enterococcus spp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. To investigate if this is a potential risk in military working dogs (MWDs), we analyzed antibiotic resistance patterns and genetic relatedness of Enterococcus spp. isolated from fecal samples of MWDs of four different age groups. Isolation rates of Enterococcus spp., Enterococcus (E.) faecalis, and E. faecium, were 87.7% (57/65), 59.6% (34/57), and 56.1% (32/57), respectively, as determined by bacterial culture and multiplex PCR. The isolation rate of E. faecalis gradually decreased with age (puppy, 100%; adolescent, 91.7%; adult, 36.4%; and senior, 14.3%). Rates of resistance to the antibiotics ciprofloxacin, gentamicin, streptomycin, sulfamethoxazole/trimethoprim, imipenem, and kanamycin among Enterococcus spp. increased in adolescents and adults and decreased in senior dogs, with some isolates having three different antibiotic resistance patterns. There were indistinguishable pulsed-field gel electrophoresis patterns among the age groups. The results suggest that Enterococcus is horizontally transferred, regardless of age. As such, periodic surveillance studies should be undertaken to monitor changes in antibiotic resistance, which may necessitate modification of antibiotic regimens to manage antibiotic resistance transmission.
