Messenger RNA differential display of rat osteoblasts treated with titanium particles
10.3760/j:issn:0529-5815.2001.01.025
- VernacularTitle:钛颗粒对大鼠成骨细胞影响的mRNA差异显示研究
- Author:
Qingjun MA
1
;
Gengting DANG
;
Takei H
;
Kim J
;
Sung Paul KL
Author Information
1. 北京大学医学部附属第三医院(北医三院)
- From:
Chinese Journal of Surgery
2001;39(1):72-75
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To observe the gene expression of rat osteoblasts treated with titanium particles and to identify the novel genes which may be related to the periprosthetic osteolysis. Methods Neonatal rat osteoblasts were grown in DME medium with 10% bovine serum, and 0.1% titanium particles were added to the medium when the cells reached 80% confluency.After 24 hours，the total RNA was isolated.Messenger RNA differential display techniques were used to compare the differences between the treated and the control group. Furthermore，the differentially displayed gene fragments were isolated，reamplified，and cloned for reverse northern hybridization，northern hybridization and sequencing. The sequences were compared with GenBank database using the BLAST network service at the National Center for Biotechnology Information（NCBI）. Results 35 fragments were differentially displayed between tho two groups. Of the 35 fragments that were analyzed by reverse northern hybridization，the 5 positive genes were sequenced and compared with NCBI.One of the five positive genes, named G46T, showed 92% homology with mouse macrophage-derived monocyte chemotactic protein-3. The other 4 positives were unknown genes. Conclusions Titanium particles may alter the gene expression of rat osteoblasts.G46T gene may be related with periprosthetic osteolysis.
