Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model.
10.4142/jvs.2017.18.2.245
- Author:
Se Eun KIM
1
;
Tae Hyun KIM
;
Shin Ae PARK
;
Won Tae KIM
;
Young Woo PARK
;
Jae Sang AHN
;
Manbok JEONG
;
Min Young KIM
;
Kangmoon SEO
Author Information
1. Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. kmseo@snu.ac.kr
- Publication Type:Original Article
- Keywords:
dog;
doxycycline;
horse chestnut leaf extract;
matrix metalloproteinase;
periodontal disease
- MeSH:
Aesculus*;
Animals;
Collagenases;
Dogs;
Doxycycline;
Gelatinases;
Horses*;
Matrix Metalloproteinases;
Peptide Hydrolases;
Periodontal Diseases;
Periodontal Ligament;
Periodontitis*;
Tooth
- From:Journal of Veterinary Science
2017;18(2):245-251
- CountryRepublic of Korea
- Language:English
-
Abstract:
Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was obtained from horse chestnut leaf and its MMP inhibitory activities estimated. Periodontitis was induced in beagles assigned to 4 groups and medicated for 6 weeks: low dose test (LT; ALH-L1005, 100 mg/kg/day), high dose test (HT; ALH-L1005, 200 mg/kg/day), positive control (PC; doxycycline, 10 mg/kg/day), or negative control (NC; placebo). Before and after administration, clinical indices of the teeth and MMP quantity in gingival tissues using zymography were measured. Clinical conditions of the LT, HT, and PC groups were significantly improved after 6 weeks. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT, and PC groups but not in the NC group. The results suggest that ALH-L1005 could be an effective agent for clinical prevention and treatment of periodontitis by inhibiting the gelatinase and collagenase activities, which can detach periodontal ligaments from alveolar bone.
