Osteoblastic related gene expression in chondrosarcoma
10.3760/j:issn:0529-5815.2001.01.021
- VernacularTitle:成骨细胞相关基因在软骨肉瘤中的表达
- Author:
Wei GUO
1
;
Healey H JOHN
Author Information
1. 北京大学医学部第二临床医学院(北京人民医院)
- From:
Chinese Journal of Surgery
2001;39(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of osteoblast-associated genes in chondrosarcomas and to identify phenotypes of tumor cell differentiation. Methods Expression of osteocalcin (OC),alkaline phosphatase (AP),osteonectin (ON) and core binding factor A1 (Cbfa1) was analyzed semi-quantitatively by RT-PCR and blotting and was correlated with histologic grade and prognosis in 32 chondrosarcoma patients. Results There were statistically significant relations between the pathologic grade and the presence of OC, AP or Cbfa1 transcripts. These osteoblast-associated genes were more often expressed in grade Ⅲ chondrosarcomas. Osteocalcin mRNA expression was detected in 9 of 12 (75%) grade Ⅲ chondrosarcomas and 4 of 20 (20%) grade Ⅰ-Ⅱ chondrosarcomas (P=0.008). AP mRNA was found in 21 of 32 chondrosarcomas,including 11 of 12 (92%) grade Ⅲ and 10 of 20 (50%) grade Ⅰ-Ⅱ chondrosarcomas (P=0.018).OC or AP transcript was associated with decreased disease free survival (P=0.016 for OC,and P=0.018 for AP, Log-rank test).Cbfa1 mRNA was only detected in 4 grade Ⅲ chondrosarcomas. ON was detected in all chondrosarcoma samples. No correlation was observed between ON transcript and pathological grade or disease free survival. Conclusions OC transcript may be detected in the majority of high grade, but in a few grade Ⅰ-Ⅱ chondrosarcomas, and is associated with poor prognosis in patients with chondrosarcoma. The difference in AP mRNA positivity between grade Ⅰ-Ⅱ and grade Ⅲ group is significant. AP expression is also related to decreased disease free survival. OC or AP expression in chondrosarcoma might be of independent prognostic value. Cbfa1 appears to have similar features with OC in that it is expressed more frequently in high grade chondrosarcomas.
