Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines.
- Author:
Jin Woo KIM
;
Mee Ran KIM
;
Jae Hoon KIM
;
Tae Eung KIM
;
Tae Chul PARK
;
Hun Young LEE
;
Seung Jo KIM
;
Sung Eun NAMKOONG
- Publication Type:Original Article
- Keywords:
Transforming growth factor-beta1;
c-myc;
Cervical cancer
- MeSH:
Blotting, Northern;
Carcinoma, Squamous Cell;
Cell Line*;
Epithelial Cells;
Genes, myc*;
Keratinocytes;
Oncogenes;
RNA, Messenger;
Skin;
Transforming Growth Factor beta;
Transforming Growth Factor beta1;
Uterine Cervical Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
1997;40(1):154-160
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Transforming growth factor-beta1(TGF-beta1) is known to be a potent growth inhibitor for many cell types, including most epithelial cells. In skin keratinocytes, TGF-beta1 has been shown to inhibit growth and to rapidly reduce c-myc expression. However, the molecular mechanism of TGF-beta1 action on cell growth of cervical carcinoma has not yet been elucidated. We thus assessed the effect of TGF-beta1 on the growth of cervical carcinoma cell lines. Two cervical squamous carcinoma cell lines were incubated with varying concentration of TGF-beta 1, and growth inhibition was evaluated with tetrazolium-based colorimetric assay. After culturing in concentrations of 0.1~10 ng/ml in both cell lines. Northern blot analysis revealed c-myc mRNA expression was suppressed by 10 ng/ml of TGF-beta 1 following 6-hour of treatment in both cell lines. These results suggest that TGF-beta1 inhibits the growth of cervical carcinoma cells by suppressing c-myc oncogene expression.
