Regulation of Inflammatory Repertoires and NF-kappaB Signal Transduction by DDB, an Active Compound from Schizandra Chinensis Baillon.
- Author:
Seong Soo JOO
1
;
Yeong Min YOO
;
Tae Joon WON
;
Min Jung KIM
;
Seon Goo LEE
;
Kwang Woo HWANG
;
Do Ik LEE
Author Information
- Publication Type:Original Article
- Keywords: Nitric oxide; iNOS; NF-KB; IL-1beta; biphenyl; dimethyl dicarboxylate; alzheimer's disease
- MeSH: Alzheimer Disease; Blotting, Western; Brain; Cues; Dementia; Gene Expression; Inflammation; Interleukin-1beta; Microglia; Neurons; NF-kappa B*; Nitric Oxide; Nitric Oxide Synthase Type II; Schisandra*; Signal Transduction*
- From:Immune Network 2006;6(1):27-32
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Chronic inflammation in the brain has known to be associated with the development of a various neurological diseases including dementia. In general, the characteristic of neuro-inflammation is the activated microglia over the brain where the pathogenesis occurs. Pro-inflammatory repertoires, interleukin-1beta (IL-1beta) and nitric oxide (NO), are the main causes of neuro-degenerative disease, particularly in Alzheimer's disease (AD) which is caused by neuronal destruction. Those pro-inflammatory repertoires may lead the brain to chronic inflammatory status, and thus we hypothesized that chronic inflammation would be inhibited when pro-inflammatory repertoires are to be well controlled by inactivating the signal transduction associated with inflammation. METHODS: In the present study, we examined whether biphenyl dimethyl dicarboxylate (DDB), an active compound from Schizandra chinensis Baillon, inhibits the NO production by a direct method using Griess reagent and by RT-PCR in the gene expression of inducible nitric oxide synthase ((i)NOS) and IL-1beta. Western blots were also used for the analysis of NF-kappaB and IkappaB. RESULTS: In the study, we found that DDB effectively inhibited IL-1beta as well as NO production in BV-2 microglial cell, and the translocation of NF-kappaB was comparably inhibited in the presence of DDB comparing those to the positive control, lipopolysaccharide. CONCLUSION: The data suggested that the DDB from Schizandra chinensis Baillon may play an effective role in inhibiting the pro-inflammatory repertoires which may cause neurodegeneration and the results imply that the compound suppresses a cue signal of the microglial activation which can induce the brain pathogenesis such as Alzheimer's disease.
