Impact of lysosome-associated protein transmembrane-4 beta on proliferation and invasion of colorectal cancer
10.3760/cma.j.issn.1671-0274.2015.06.022
- VernacularTitle:溶酶体相关4次跨膜蛋白B对结直肠癌增殖和侵袭的影响
- Author:
Yang LUO
1
;
Zhengqian BIAN
;
Guangyao YE
;
Minhao YU
;
Zhengshi WANG
;
Shaolan QIN
;
Yifei MU
;
Ming ZHONG
Author Information
1. 200127,上海交通大学医学院附属仁济医院胃肠外科
- Keywords:
Colorectal neoplasms;
Lysosome-associated protein transmembrane-4 beta;
HCT116 cells;
Proliferation;
Invasion
- From:
Chinese Journal of Gastrointestinal Surgery
2015;(6):606-610
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether lysosome-associated protein transmembrane-4 beta (LAPTM4B) over-expression is associated with the proliferation and invasion in colorectal cancer (CRC). Methods Thirty pairs of CRC tissues, containing carcinoma and adjacent tissues, were used for the examination of LAPTM4B mRNA expression by real-time quantitative PCR (qPCR) assays. Then immunohistochemistry was performed to examine LAPTM4B protein expression in 6 pairs of CRC tissues. Over-expression LAPTM4B and low-expression LAPTM4B cell models were constructed with HCT116 CRC cell lines. CCK8 assay was used to detect the proliferation and Transwell assay was used to detect the invasion of the model cells. Results qPCR and immunohistochemistry results showed that LAPTM4B expression levels in CRC were higher compared to adjacent tissues (all P<0.01). CCK8 and Transwell assays results showed that LAPTM4B promoted proliferation and invasion of HCT116 cell lines model cells (all P<0.01). Conclusion LAPTM4B promotes the proliferation and invasion in CRC patients, and may be used as an important potential marker.
