Co-inhibition effect of 17-DMAG and oxaliplatin on proliferation and invasion of colorectal cancer cells
10.3760/cma.j.issn.1671-0274.2015.04.018
- VernacularTitle:17-DMAG与奥沙利铂对结直肠癌细胞增殖和侵袭的协同抑制作用
- Author:
Jianping ZHOU
1
;
Weimin WANG
;
Jianliang DENG
;
Yan ZHOU
;
Lulu WU
;
Zhiyuan GUO
;
Jianneng SHI
;
Jun SHI
;
Sujun ZHOU
;
Zekuan XU
Author Information
1. 214200,江苏大学附属宜兴医院胃肠外科
- Keywords:
Colorectal neoplasms;
Heat shock protein;
Proliferation;
Metastasis
- From:
Chinese Journal of Gastrointestinal Surgery
2015;(4):370-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of heat shock protein 90 (HSP90) inhibitor (17-DMAG) and oxaliplatin on the proliferation and invasion of colorectal cancer. Methods After 17-DMAG, oxaliplatin and half-dose combination of 2 drugs processing colorectal cancer SW480 and HCT116 cell lines, CCK8 assay was applied to detect cell viability. RT-PCR and Western blot were used to detect the expression level of the apoptosis-related molecules. Transwell chemokine axis experiment and Western blot were employed to detect cell invasion ability and the expression level of tumor metastasis-associated protein. Results The growth of SW480 and HCT116 cells was inhibited after the administration of 17-DMAG and oxaliplatin (P<0.05) in dose- and time-dependent manner. Processed by 17-DMAG 100 nmol/L, oxaliplatin 50 mg/L and half-dose combination of 2 drugs, transcription level of the apoptosis inhibitory gene (Bcl-2) in SW480 and HCT116 cells was decreased, the level of apoptosis promoting gene (Bax) transcription and protein PARP-1 spliceosome expression was increased, and the above trend was more obvious when using half-dose combination of 2 drugs. Transwell chemokine axis experiments showed the penetrating relative percentage and expression level of MMP9 and integrin β3 decrease d, especially for half-dose combination of 2 drugs. Conclusion 17-DMAG and oxaliplatin can co-inhibit the proliferation and invasion of colorectal cancer.
