Relationship between CD4+CD25HighCD127low regularly T cells in the peripheral blood and tumor regression after neoadjuvant therapy in patients with rectal cancer
10.3760/cma.j.issn.1671-0274.2015.04.016
- VernacularTitle:直肠癌新辅助治疗后外周血调节性T细胞水平与肿瘤缓解的关系
- Author:
Haibo WANG
1
;
Haiyi LIU
;
Wen SU
;
Yixun ZHANG
;
Yi FENG
;
Yanjun LU
Author Information
1. 山西医科大学附属肿瘤医院结直肠外科
- Keywords:
Rectal tumor;
Neoadjuvant therapy;
Tumor regression;
Regulatory T cells
- From:
Chinese Journal of Gastrointestinal Surgery
2015;(4):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the proportion change of immune cells in the peripheral blood of patients with rectal cancer after neoadjuvant therapy and to explore the relationship between tumor regression and CD4 +CD25HighCD127low regularly T cells (Treg cells). Methods Patients with rectal cancer who underwent the neoadjuvant therapy before surgery at the Shanxi Cancer Hospital Colorectal Surgery Department from January to December 2013 were prospectively enrolled. These patients were divided into down-staging group and non-down-staging group according to the change of staging in accordance with TNM classification for rectal cancer after neoadjuvant therapy. Flow cytometry was used to analyze the proportions of Treg cells, CD4+T cells, CD8+T cells, NK cells, B cells, and CD4+/CD8+ratio in the peripheral blood from these patients before and after neoadjuvant therapy. Results A total of 108 patients were enrolled, including 76 cases in the down staging group and 32 cases in the non-down-staging group. Differences of immune cells proportions between two groups before neoadjuvant therapy were not statistically significant(all P>0.05). In the down-staging group, the proportions of Treg cells, B cells and CD4+/CD8+ ratio were decreased while the proportion of NK cells did not change obviously after the neoadjuvant therapy. Interestingly, in the non-down-staging group, the proportions of B cells and CD4+/CD8+ ratio were decreased while the proportions of Treg cells and NK cells did not change obviously after the neoadjuvant therapy. In addition, after neoadjunvat therapy, the proportion of Treg cells in down-staging group was significantly lower than that in non-down-staging group [(4.4±1.7)% vs. (6.2±1.9)%, P=0.001]. Conclusion For patients in the down-staging group after neoadjuvant therapy, the proportion of Treg cells in peripheral blood decreases, suggesting that Treg cells may be a valuable biomarker for assessing tumor regression.
