Expression of vasohibin-1 in colorectal cancer tissue and its correlation with vascular endothelial growth factor A and microvessei density
10.3760/cma.j.issn.1671-0274.2015.03.016
- VernacularTitle:结直肠癌组织中血管抑制蛋白1表达的临床意义及其与血管内皮生长因子A和微血管密度的相关性
- Author:
Ganggang SHI
1
;
Jingpeng HAO
;
Li WANG
;
Bin HE
;
Jianchen GAN
;
Hui WANG
;
Rongzeng SUO
Author Information
1. 300211,天津医科大学第二医院肛肠外科
- Keywords:
Colorectal neoplasms;
Vasohibin-1;
Vascular endothelial growth factor A;
Microvessel density
- From:
Chinese Journal of Gastrointestinal Surgery
2015;(3):272-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance of vasohibin-1 expression in colorectal cancer tissues and its correlation with vascular endothelial growth factor A ( VEGF-A ) and microvessel density (MVD). Methods Tumor tissues and paired adjacent normal tissue (distance to cancer >5 cm) from 60 colorectal cancer patients undergoing resection in the Second Hospital of Tianjin Medical University from June 2013 to November 2013 were included in this study. The protein expressions of vasohibin-1, VEGF-A and MVD were detected by immunohistochemical staining. The mRNA expressions of vasohibin-1 and VEGF-A were detected by RT-PCR. The protein expressions of vasohibin-1 and VEGF-A were observed by Western blot. Correlation among parameters was examined. Results Vasohibin-1 expression was mainly localized in the cytoplasm of tumor cells and endothelial cells. VEGF-A expression was mainly localized in the cytoplasm and membrane of tumor cells. The expressions of vasohibin-1, VEGF-A and MVD in colorectal tumor tissues were significantly higher than those in corresponding adjacent tissues [43.3%(26/60) vs. 16.7%(10/60), 51.7%(31/60) vs. 18.3%(11/60), (39.67±16.80)/mm2 vs. (17.85±6.43)/mm2, all P<0.05]. Higher vasohibin-1 expression was significantly associated with TNM stage and metastasis (P<0.05). Vasohibin-1 expression was positively correlated with VEGF-A and MVD (r=0.378, 0.628, all P<0.05). Vasohibin-1 and VEGF-A mRNA expressions and protein expressions in colorectal cancer tissues were significantly higher than those in corresponding adjacent tissues (all P<0.05). Conclusion Vasohibin-1 expression in colorectal cancer tissues is significantly higher as compared to corresponding adjacent tissues. Vasohibin-1 expression is positively correlated to VEGF-A and MVD, and associated to TNM stage and metastasis. Positive vasohibin-1 expression indicates a poor prognosis of patients with colorectal cancer.
