Anti-tumor effect of 5-FU-PLLA-CNTs on human gastric carcinoma cell lines in vitro
10.3760/cma.j.issn.1671-0274.2014.04.020
- VernacularTitle:载氟尿嘧啶聚乳酸碳纳米管复合材料对胃癌细胞株的体外杀伤效应
- Author:
Jun GU
1
;
Maolan LI
;
Xiangsong WU
;
Wenguang WU
;
Lin ZHANG
;
Qichen DING
;
Jiahua YANG
;
Hao WENG
;
Qian DING
;
Runfa BAO
;
Yijun SHU
;
Yingbin LIU
Author Information
1. 200092,上海交通大学医学院附属新华医院普通外科 普通外科实验室 上海交通大学医学院胆道疾病研究所
- Keywords:
Stomach neoplasms;
Fluorouracil;
Poly-L-lactic acid;
Anti-tumor effects
- From:
Chinese Journal of Gastrointestinal Surgery
2014;(4):383-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare cisPLLAtin-loaded polylactic acid/cnts , and to study the anti-tumor effect of 5-FU-PLLA-CNTs on human gastric carcinoma cell lines (MGC803 and MNK45). Methods 5-FU-PLLA-CNTs were prepared with ultrasound emulsification. The morphology of 5-FU-PLLA-CNTs was determined by scanning electron microscope (SEM), and its drug loading and drug release curve in vitro were detected by UV-Vis-NIR spectrophotometer. Cells were divided into experiment, positive control and negative control groups. CCK8 method was used to test the cytotoxic effect of 5-FU-PLLA-CNTs in different concentrations on MGC803 and MNK45 cell proliferation. Flow cytometry was employed to measure the apoptotic rate of MGC803 and MNK45 cells before and after the intervention of 5-FU-PLLA-CNTs. Results Deep layer film of 5-FU-PLLA-CNTs was successfully established, whose drug-load rate was (4.54 ±0.43)%, entrapment rate was (21.56 ±2.36)%. In vitro release test showed release rate within 24 h of 5-FU-PLLA-CNTs was 23.9% in a aslowly increasing manner, and accumulating release rate was 85.3% at day 31. CCk8 experiment revealed , as compared to control group, 5-FU-PLLA-CNTs significantly inhibited the proliferation of two cell lines in dose-dependent and time-dependent manner. The best 5-FU-PLLA-CNTs concentration of inhibition for human gastric cancer cell lines was 1 mg/well. Flow cytometry indicated the apoptotic rate of MGC803 and MNK45 cells in experiment group treated by 1 mg/well 5-FU-PLLA-CNTs significantly increased as compared to negative control group (P<0.05), while the difference was not significant as compared to positive control group (P>0.05). Conclusion The 5-FU-PLLA-CNTs has good drug sustained-release capacity, and can significantly kill and inhibit the proliferation of MGC803 and MNK45 cell lines.
