Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and alpha-Galactosidase: A Comparative Study.
10.5090/kjtcs.2012.45.6.368
- Author:
Byoung Ju MIN
1
;
Yong Jin KIM
;
Jae Woong CHOI
;
Sun Young CHOI
;
Soo Hwan KIM
;
Hong Gook LIM
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, Korea.
- Publication Type:Comparative Study ; Original Article
- Keywords:
Bioprosthesis;
Decellularization;
Glutaraldehyde;
alpha-Gal
- MeSH:
alpha-Galactosidase;
Bioprosthesis;
Compliance;
Fatigue;
Glutaral;
Pericardium;
Permeability;
Sodium;
Sprains and Strains;
Tensile Strength
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2012;45(6):368-379
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. alpha-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with alpha-galactosidase. MATERIALS AND METHODS: Bovine pericardial tissues were allocated into three groups: fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with alpha-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test. RESULTS: Decellularization and elimination of alpha-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with alpha-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001). CONCLUSION: Favorable decellularization and elimination of alpha-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of alpha-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate ioprosthesis with decellularization and elimination of alpha-gal including favorable mechanical properties too.
