Relationships of expression of high mobility group box 1 protein and toll-like receptor 4 in patients with clinical characteristics of refractory epilepsy and their predictive value
- VernacularTitle:高迁移率族蛋白B1、Toll样受体4表达与难治性癫痫患者临床特征的关系及其预测价值
- Author:
Yu WANG
1
;
Jixue YANG
;
Dianqu GUO
;
Wenbin LIU
Author Information
- Keywords: toll-like receptor 4; high mobility group box 1 protein; prediction; refractory epilepsy; correlation; retrospective analysis
- From: Journal of Clinical Medicine in Practice 2024;28(19):68-72,83
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the correlations of the expression of high mobility group box 1 protein(HMGB1)and toll-like receptor 4(TLR4)with the clinical characteristics of patients with refractory epilepsy,as well as their predictive value.Methods A retrospective analysis was conducted on the clinical data of 84 patients with refractory epilepsy,and they were included in ob-servation group.During the same period,35 patients with intracranial hypertension undergoing crani-ectomy for decompression were selected as control group.Immunohistochemistry was used to detect the expressions of HMGB1 and TLR4,while Nissl staining was applied to observe tissue morphology.The relevant experimental results were analyzed simultaneously.Results The expression levels of HMGB1 and TLR4 in the observation group were significantly higher than those in the control group(P＜0.05).The optical density values of HMGB1 and TLR4 protein bands as well as internal refer-ence β-actin bands in the observation group were significantly higher than those of the control group(P＜0.05).The intensity of TLR4 expression was correlated with seizure frequency,seizure dura-tion,disease duration,and seizure type,while the intensity of HMGB1 expression was correlated with seizure frequency,seizure duration,and disease duration.In the lesion tissues,the expression of TLR4 was positively correlated with the expression of HMGB1,suggesting a synergistic effect between them.Receiver Operating Characteristic(ROC)curve analysis revealed that the areas under the curve(AUCs)for TLR4 and HMGB1 proteins in predicting refractory epilepsy were 0.888 and 0.923,respectively.Conclusion HMGB1 and TLR4 are highly expressed in the lesions of patients with refractory epilepsy,and their expression intensities are positively correlated.They can predict the occurrence and development of the disease to a certain extent,providing new biomarkers for the di-agnosis and treatment of refractory epilepsy.
