Effect of ANGPTL 4 on M2 macrophages differentiation

VernacularTitle:血管生成素样蛋白4对M2型巨噬细胞分化的影响
Author: Dandan WU ¹ ; Shizhen DING ; Guotao LU ; Weiming XIAO ; Weijuan GONG
Author Information

1. 扬州大学医学院免疫学教研室
Keywords: angiopoietin-like 4; M2 macrophage; tumor immune escape; differentiation
From: Journal of Clinical Medicine in Practice 2018;22(9):1-5
CountryChina
Language:Chinese
Abstract: Objective To analyze the effect of angiopoietin-like protein 4(ANGPTL4) on M2 macrophage differentiation.Methods The frequency of M2 macrophages in spleen of ANGPTL 4-/-mice and the controls was detected by flow cytometry.And the changes of M2 macrophages was measured by LPS stimulation.F4/80 + macrophages was separated by flow cytometry and treated with LPS or recombinant ANGPTL 4 protein.The secretion of IL-4 in CD4 + T cells was observed after purified macrophages after co-culture with CD4 + T cells by the flow-cytometric intracellular staining method.Results There was no significant difference in the frequency of M2 macrophages in ANGPTL 4-/-mice and controls.LPS stimulation did not affect the expression of M2 macrophages from ANGPTL 4-/-mice.The macrophages from ANGPTL 4/-mice did not promote differentiation of CD4 + T cells into Th2 cells.After co-culturing of macrophages and CD4 + T cells for 48 h in vitro,IL-4 secretion of CD4 + T cells was not changed.Conclusions ANGPTL4 has no effects on M2 macrophage differentiation.