Preparation and application of a new type of magnetic fluorescent nanomaterials
10.7619/jcmp.201805029
- VernacularTitle:一种新型磁性荧光纳米材料的制备与应用分析
- Author:
Guangqin ZHANG
1
;
Hong ZHANG
;
Li WANG
Author Information
1. 武汉大学人民医院药学部
- Keywords:
magnetic nanoparticles;
fluorescent;
napH thalimide;
rhodamine B;
pH;
fluorescent probe
- From:
Journal of Clinical Medicine in Practice
2018;22(5):94-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a new type of magnetic fluorescent nanoparticles and characterize them and analyze their applications in the field of biology and medicine.Methods In this paper,magnetic fluorescent nanoparticles-Fe3O4@PEI@RN were prepared by chemical synthesis.The content of the main components of the prepared particles was measured.The particles' size was analyzed by dynamic light scattering analysis,the effect of pH on the fluorescence properties was measured by fluorescence analysis,and some common metal ions,proteins and DNA were investigated whether they would interfere the particles'properties.Results The content of Fe3O4@PEI@RN showed that the autom of Fe was 14.1 mg/mL,rhodamine B was 7.6 mg/mL and napH thalimide was 94.8 mg/mL.The dynamic light scattered that the average particle diameter of the magnetic fluorescent nanoparticles was 224 nm.Fluorescence analysis showed that the fluorescence ratio F of Fe3O4@PEI@RN at 532 nm and 574 nm were pH-sensitive with a pKa of 6.73.When the concentration of common metal ions was 5 × 10-6,they did not change their fluorescence determinations,and when the protein concentration was less than 14 mg/mL and the DNA concentration was less than 20 mg/mL,they did not interfere with their determinations.Conclusion A new type of magnetic fluorescent nanomaterials is successfully prepared.Its good fluorescence shows it has great potential in the field of biomedicine.
