Expressions of peripheral blood lymphocyte and serum cytokine in children with secretory otitis media and effect of hormone intervention
10.7619/jcmp.201803014
- VernacularTitle:分泌性中耳炎患儿外周血淋巴细胞及血清细胞因子的表达及激素干预效果
- Author:
Yongjun FENG
1
;
Mingjing WANG
;
Mengying LYU
;
Qihua DENG
;
Xiangming WU
;
Chunrong ZENG
;
Zhiming WU
Author Information
1. 海南医学院第二附属医院耳鼻咽喉头颈外科
- Keywords:
secretory otitis media;
children;
peripheral blood;
lymphocyte subgroups;
cytokines;
glucocorticoids therapy
- From:
Journal of Clinical Medicine in Practice
2018;22(3):51-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expressions of lymphocytes in the peripheral blood and serum cytokines in the children with secretory otitis media (SOM) and the effects of glucocorticoids interventions.Methods Totally 90 SOM children were selected as case group,and 30 healthy children were selected as control group.The case group was randomly divided into group A (simple oral antibiotic treatment),group B (oral antibiotics combined with local glucocorticoid treatment) and group C (oral antibiotics combined with systemic glucocorticoid treatment),30 cases in each group.The CD4 + T lymphocytes percentage,CD8 + T lymphocytes percentage,the ratio of CD4 +/CD8 + T lymphocytes in peripheral blood and the serum interleukin-2 (IL-2),interferon gamma (IFN-γ),tumor necrosis factor alpha (TNF-α),interleukin-6 (IL-6),interleukin-10 (IL-10) levels were detected and compared between case group and control group.The air conduction auditory thresholds under different frequency of the patients in group A,group B and group C were examined and compared.Results The CD4 + T lymphocytes percentage,the ratio of CD4 +/CD8 + T lymphocytes in peripheral blood and the serum IL-2,IFN-γ,TNF-α,IL-6,IL-10 levels of the patients in the case group were significantly higher than those in the control group (P ＜ 0.0 5).After the treatment,the above indicators of the children in group A,group B and group C decreased,and there were significant differences before and after the treatment between two groups (P ＜ 0.05).The air conduction auditory thresholds under different frequency of the patients in group B or group C were significantly lower than those in group A (P ＜ 0.05),while there were no significant differences in the air conduction auditory thresholds under different frequency between the children in group B and group C (P ＞ 0.05).Conclusion The patients with SOM show imbalanced cell immune function and cytokines expressions in the peripheral blood.The combination of glucocorticoids therapy and routine antibiotic therapy can effectively improve the immune function and the therapeutic effects.
