Study on the regulation mechanism of specific proteins c-kit of interstitial cells of Cajal in the pathogenesis of slow transit constipation in rats
10.7619/jcmp.201723013
- VernacularTitle:大鼠慢传输型便秘发病的Cajal间质细胞特异性蛋白c-kit-SCF调控机制研究
- Author:
Zhihao LIU
1
;
Shuguang LI
;
Xiangyang YU
;
Jianli CHEN
;
Wenbin CAO
;
Yongkui ZHAO
;
Guozhi ZHANG
Author Information
1. 河北省唐山市开滦总医院范各庄医院普通外科
- Keywords:
slow transit constipation rats;
fluorescent quantitative PCR;
inflammatory reaction;
stem cell factor ligands
- From:
Journal of Clinical Medicine in Practice
2017;21(23):43-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective The explore regulation mechanism of specific proteins c-kit of interstitial cells of Cajal in the pathogenesis of slow transit constipation in rats.Methods Slow transit constipation in rat model was duplicated by injections of morphine (25 mg/kg,45 d).The number and weight of fecal granule were assayed.Daily average number of fecal particles,quality,and the time for first black discharge were analyzed.At the same time,the c-kit and SCF expression were detected by Real-time PCR and Western blot.The changes of inflammatory factors such as IL-1β,IL-2 and IL-10 were also detected by ELISA.Results Daily average number of fecal particles in constipation group was decreased,quality was higher,and the first time for first black discharge was prolonged,which showed significant differences (P ＜ 0.05).The expression levels of c-kit and SCF were decreased significantly,and levels of inflammatory factors IL-1β,IL-2 and IL-10 were higher in constipation rats than the controls (P ＜ 0.05).Conclusion The pathogenic progress of slow transit constipation rats are regulated,which is also related with inflammatory reactions.
