Detection of ZnT-8A, IA-2A, IAA, GADA and ICA and 3-year onset risk of direct relatives of patients with type 1 diabetes mellitus
10.7619/jcmp.201717008
- VernacularTitle:T1DM患者直系亲属ZnT-8A、IA-2A、IAA、GADA、ICA检测及3年发病风险分析
- Author:
Yanqing LI
1
;
Zijie DONG
;
Xiaohui SUN
;
Hongbin ZHAO
Author Information
1. 河北省石家庄市第一医院检验科
- Keywords:
T1DM : direct relatives;
autoantibodies;
onset risk
- From:
Journal of Clinical Medicine in Practice
2017;21(17):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of detection of ZnT-8A,IA-2A,IAA,GADA and ICA and 3-year onset risk in direct relatives of patients with type 1 diabetes mellitus (T1DM).Methods The positive rates of serum zinc transporter 8 antibody (ZnT-8A) in T1DM patients (n =80),their direct relatives (n =100) and normal subjects (n =100) were detected by radioimmunoprecipitation assay.The islet cell antibody (ICA),glutamic acid decarboxylase antibody (GADA),protein tyrosine phosphatase autoantibody (IA-2A) and insulin autoantibody (IAA) were detected by Western Blot.The combined detection efficacy of different antibodies was evaluated with ROC curve and the 3-year onset of direct relatives was analyzed.Results The antibodies detection rates of serum ZnT-8A,IA-2A,IAA,GADA and ICA were significantly higher in T1DM group than direct relatives group and healthy group (P ＜ 0.05),and the detection rates of above antibodies were significantly higher in direct relatives group than the healthy group (P ＜ 0.05).The highest total positive rate detected by the five antibodies was 91.25％,and the highest total positive rates detected by the combination of 2,3 and 4 antibodies were 83.75％,87.5％ and 90.0％,respectively.The area under ROC (AUC) of the 5 antibodies was the largest but there was no significant difference in AUC among different combinations of antibodies.All positive rates detected by combined antibodies were significantly higher than that of detection of ZnT-8A antibody alone (P ＜ 0.05).During 3-year follow-up,direct relatives with ZnT-8A and GADA were diagnosed with T1DM,and there were no symptoms in the other individuals.Conclusion The combined detection of 5 antibodies can improve the diagnosis rate of T1DM.The combined detection of Zn T-8A and GADA is of great value in timely and accurate classification of diabetes mellitus,and they also can be used as indicators for epidemiological screening of direct relatives of T1DM patients.
