Methylation and mRNA expression of Wif-1 gene in renal cell carcinoma
10.7619/jcmp.201715020
- VernacularTitle:肾细胞癌中Wif-1基因的甲基化状态及表达
- Author:
Xiaochen NI
1
;
Aili ZHANG
;
Shufei WEI
Author Information
1. 河北医科大学第四医院 泌尿外科
- Keywords:
renal cell carcinoma;
Wnt inhibitory factor-1;
methylation;
expression
- From:
Journal of Clinical Medicine in Practice
2017;21(15):74-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the methylation of Wif-1 gene promoter and its mRNA expression level in renal cell carcinoma, and to explore its possible mechanisms in the development of renal cell carcinoma.Methods The methylation-specific polymerase chain reaction (MSP) and reverse transcription-polymerase chain reaction (RT-PCR) were applied to investigate the methylation status of Wif-1 gene promoter and its mRNA expression levels in renal cell carcinoma (RCC) and normal kidney tissue.Results The percentage of methylation of Wif-1 gene in RCC tissue was significantly higher than that in the corresponding normal kidney tissue (P<0.05).The relative expression quantity of Wif-1 mRNA in RCC tissue was significantly lower than that in corresponding normal kidney tissue (P<0.05).In the RCC tissue, the relative expression of mRNA in the Wif-1 methylation positive group showed no significant difference with that in the methylation negative group.Conclusion Hypermethylation of the Wif-1 promtor in RCC is a frequent phenomenon.
