Effect of Gab2 on extracellular adhesion of colon cancer cells
10.7619/jcmp.201715016
- VernacularTitle:Gab2对结肠癌细胞细胞外黏附作用的影响
- Author:
Yuhui LIU
1
;
Qingqing SUN
;
Fang GUO
Author Information
1. 沈阳军区总医院 肿瘤科
- Keywords:
colon cancer;
Gab2;
extracellular adhesion
- From:
Journal of Clinical Medicine in Practice
2017;21(15):61-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Gab2 on extracellular adhesion of colon cancer cells.Methods SW620 and HCT116 were selected to establish the stable cell lines of Gab2 high expression and Gab2 low expression in two colon carcinoma cell lines.The experiment NOD/SCID mice were divided into 3 groups, 10 rats in each group.The control group included scr/MGC803 inoculation of colon cancer cells, Gab2 reduction group included Gab2 colon cancer cells with reduced inoculation of siGab2/MGC803, and elevated Gab2 group included Gab2/MGC803 colon cancer cells with elevated Gab2 inoculation.Cell migration, tumor size, invasion and cell apoptosis were observed.Results After the treatment, cells transference number calculation results showed that cell migration in GAB2 reduced group was significantly lower than that in GAB2 increased group and control group (P<0.05).NOD/SCID mice were sacrificed 40 days after inoculation, in the comparison of weight of mice before and after inoculation, the Gab2 decreased group was significantly lower than the Gab2 increased group and the control group, and the control group was significantly lower than the Gab2 increased group.In comparison of tumor volume, Gab2 increased group were significantly higher than the Gab2 decreased group and the control group.In Gab2 decreased group, tumor volume inhibition rate was significantly higher than the control group (P<0.05).The correlation analysis showed that MMP-2 and MMP-9 protein expression were positively correlated with GAB2 protein expression.Conclusion Gab2 can effectively regulate the number of cell migration in vitro, significantly inhibit the invasion and metastasis of colon cancer cells in mice, and inhibit the growth and metastasis of colon cancer by regulating the expression of MMP-2 and MMP-9 protein.
