Expression and clinical significance of miRNA-146a in CD14+ MC of children with sepsis
10.7619/jcmp.201709031
- VernacularTitle:脓毒症患儿CD14+单核细胞中miR-146a的表达及其临床意义
- Author:
Yuhui WU
1
;
Ying QI
;
Weike MA
;
Yuzheng LI
;
Weiguo YANG
;
Chengrong LI
Author Information
1. 广东省深圳市儿童医院 PICU
- Keywords:
children;
miR-146a;
sepsis;
CD14+ monocytes
- From:
Journal of Clinical Medicine in Practice
2017;21(9):119-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR-146a in CD14+ monocytes (MC) of children with sepsis.Methods Forty children with sepsis in PICU were enrolled in the study.Fifteen children with systemic inflammatory response syndrome (SIRS) afteRsurgical treatment and fifteen healthy children were selected as control group.Expressions of miR-146a and TNF-α,IL-10 of CD14+ MC were detected by real-time quantitative PCR(qRT-PCR).HLA-DRlevels of CD14+ MC were detected by flow cytometry.Levels of high-sensitivity C-reactive protein (CRP) and procalcitonin (PCT) were detected.Results The HLA-DRlevels of CD14+ MC in all the children with sepsis were oveR30%.The miR-146a level of CD14+ MC were significantly higheRin sepsis group than SIRS group and healthy controls (P<0.05),and the level decreased when sepsis children were recovered (P<0.05).The TNF-α,IL-10 level of CD14+ MC in children with sepsis was significantly higheRthan healthy controls (P<0.05).Conclusion The expressions of CD14+ MC miR-146a in children with sepsis are significantly up-regulated.
