Evaluation of osteogenic activity of periosteal-derived cells treated with inflammatory cytokines.
10.5125/jkaoms.2010.36.5.341
- Author:
Bong Wook PARK
1
;
Mun Jeoung CHOI
;
Young Sool HAH
;
Hee Young CHO
;
Deok Ryong KIM
;
Uk Kyu KIM
;
Hee Jea KANG
;
Jong Ryoul KIM
;
June Ho BYUN
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea. surbyun@gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
Periosteal-derived cells;
Inflammatory cytokine;
Tumor necrosis factor-alpha (TNF-alpha);
Interleukin-1beta (IL-1beta)
- MeSH:
Alkaline Phosphatase;
Anthraquinones;
Ascorbic Acid;
Bone Resorption;
Cell Culture Techniques;
Cytokines;
Dexamethasone;
Durapatite;
Glycerophosphates;
Homeostasis;
Humans;
Interleukins;
Osteoblasts;
Osteoclasts;
Osteogenesis;
Periosteum;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2010;36(5):341-345
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-alpha with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1beta with different concentrations (0.01, 0.1, and 1 ng/mL) were added. RESULTS: Both TNF-alpha and IL-1beta stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-alpha and IL-1beta increased the level of ALP expression in a dose-dependent manner. Both TNF-alpha and IL-1beta also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. CONCLUSION: These results suggest that inflammatory cytokines TNF-alpha and IL-1beta can stimulate the osteoblastic activity of cultured human periosteal-derived cells.
