RT-PCRdetection foRpneumocystis jirovecii in phlegm of patients with HIV-negative hypoimmunity
10.7619/jcmp.201709018
- VernacularTitle:HIV阴性免疫力低下患者痰液中耶氏肺孢子的实时荧光定量PCR检测
- Author:
Xuan SHE
1
;
Yunqing YAO
;
Chuan ZHAO
Author Information
1. 四川省遂宁市中心医院 感染科
- Keywords:
RT-PCR;
diagnosis;
pneumocystis pneumonia;
HIV-negative hypoimmunity
- From:
Journal of Clinical Medicine in Practice
2017;21(9):69-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of RT-PCRin diagnosis of patients with HIV-negative hypoimmunity and pneumocystis pneumonia (PCP).Methods Totally 71 phlegm samples of pulmonary infection patients with suspected PCP and HIV-negative hypoimmunity were selected.ITS-nested PCRand ITS-real-time fluorescence quantitative PCRwere used to detect pneumocystis jiroveci in samples.Results Among the 71 patients,18 cases were diagnosed as PCP.There were 25 cases with positive results by n-PCR,but the false-positive rate was 36.00%,and sensitivity,specificity and positive predictive values of n-PCRwere 88.89%,83.01% and 64.00%,respectively.If the threshold was 103 copies/mL,the specificity and positive predictive value of RT-PCRwere significantly higheRthan n-PCR.In patients with PCP,the the gene load of pneumocystis by RT-PCRwas significantly higheRthan non-PCP patients.Conclusion In patients with HIV-negative hypoimmunity,RT-PCRis more accurate and rapid than nested PCRin the diagnosis of PCP,and it can distinguish the infection and colonization of pneumocystis.
