The effect of silk fibroin and rhBMP-2 on bone regeneration in rat calvarial defect model.
10.5125/jkaoms.2010.36.5.366
- Author:
Jeong Hun NAM
1
;
Kyung Lok NOH
;
Eun O PANG
;
Woo Geun YU
;
Eung Sun KANG
;
Hae Yong KWEON
;
Seong Gon KIM
;
Young Ju PARK
Author Information
1. Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Korea. kilhyun73@naver.com
- Publication Type:Original Article
- Keywords:
Silk;
Fibroins;
Recombinant human bone morphogenetic protein-2;
Bone regeneration;
Tissue scaffolds
- MeSH:
Adult;
Alkaline Phosphatase;
Animals;
Bone Morphogenetic Protein 2;
Bone Regeneration;
Female;
Fibroins;
Humans;
Inflammation;
Osteogenesis;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins;
Silk;
Tissue Scaffolds;
Transforming Growth Factor beta;
Transplants
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2010;36(5):366-374
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION: This study evaluated the capability of silk fibroin (SF) and recombinant human bone morphogenetic protein-2 loaded SF (SF-BMP) as a bone defect replacement matrix when grafted in a calvarial bone defect of rats in vivo. MATERIALS AND METHODS: A total 70 calvarial critical size defects (5.0 mm in diameter) made on 35 adult female Sprague-Dawley rats were used in this study. The defects were transplanted with (1) rhBMP-2 loaded silk fibroin graft (SF-BMP: 0.8+10 microg), (2) Silk fibroin (SF: 10 microg), and (3) no graft material (Raw). The samples were evaluated with soft x-rays, alkaline phosphatase activity, calcium/phosphate quantification, histological and histomorphometric analysis at postoperative 4 and 8 weeks. RESULTS: The SF-BMP group (48.86+/-14.92%) had a significantly higher mean percentage bone area than the SF group (24.96+/-11.01%) at postoperative 4 weeks.(P<0.05) In addition, the SF-BMP group (40.01+/-12.43%) had a higher % bone area at postoperative 8 weeks than the SF group (33.26+/-5.15%). The mean ratio of gray scale levels to the host bone showed that the SF-BMP group (0.67+/-0.08) had a higher mean ratio level than the SF group (0.61+/-0.09) at postoperative 8 weeks. These differences were not statistically significant.(P=0.168 and P=0.243, respectively) CONCLUSION: The rhBMP-2 loaded silk fibroin graft revealed fewer immunoreactions and inflammation as well as more new bone formation than the pure silk fibroin graft. Therefore, silk fibroin may be a candidate scaffold for tissue engineered bone regeneration.
