Effect of oxaliplatin on expression of drug resistant gene in DNA damage repair in human colon cancer cells
10.7619/jcmp.201701001
- VernacularTitle:奥沙利铂对结肠癌细胞DNA损伤修复类抗药基因表达的影响
- Author:
Xiao FANG
1
;
Hua YIN
;
Tenghui HU
;
Shiguang JIN
;
Daxin WANG
Author Information
1. 扬州大学医学院
- Keywords:
oxaliplatin;
colon cancer cells;
DNA damage repair;
drug resistance
- From:
Journal of Clinical Medicine in Practice
2017;21(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of oxaliplatin on expression of drug resistant gene in DNA damage repair in human colon cancer cells.Methods The human colon cancer cell line HCT116 was processed with oxaliplatin for 48 hours.Western blot was used to examine DNA damage induced by oxaliplatin treatment.CCK-8 assay was used to explore the effect of oxaliplatin on growth of HCT116.The real-time PCR was used to detect the expression of DNA repair genes after oxaliplatin treatment.Results After the treatment of oxaliplatin,the expression of the DNA-damage-marker gene γ-H2AX increased significantly,and DNA damage excision repair related gene XPC increased significantly as well.The mRNA expression of other DNA damage repair genes had no significant increase.Conclusion The gene XPC might play a key role in generating drug resistance when treating colon cancer with oxaliplatin in clinic.
