Effects of β-carboline of alkaloids on proliferation of SGC-7901 of gastric cancer cells in human
10.7619/jcmp.201416004
- VernacularTitle:β-咔啉类生物碱对人胃癌细胞SGC-7901增殖的影响
- Author:
Yuxiang FAN
1
;
Fanye ZENG
;
Wenting HE
;
Hongliang ZHANG
Author Information
1. 新疆医科大学附属中医医院 肿瘤二科
- Keywords:
β-Carboline alkaloids;
SGC-7901;
proliferation;
apoptosis
- From:
Journal of Clinical Medicine in Practice
2014;(16):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of β-Carboline of alkaloids on proliferation of SGC-7901 of human gastric cancer cells.Methods SGC-7901 cells were cultured in vitro initially. After SGC-7901 cells were incubated with β-Carboline alkaloids at different concentrations of 5,10, 20,40 μg/mL for 24,48 hours,the inhibited proliferation rate of SGC-7901 cells were examined by Methtl Thiazolyl Tetrazolium(MTT)assay.Morphological changes of SGC-7901 cells were observed by Hoechst 33258 under fluorescence microscope.Flow cytometry was used to detect cell apoptosis after SGC-7901 cells were incubated with β-Carboline alkaloids for 24 hours.Cell gemonic DNA was detec-ted by agarose electrophoresis.Results β-Carboline of alkaloids induced damage of SGC-7901 cell in a concentration dependent manner .The IC 5 0 of β-Carboline alkaloids at 2 4 ,4 8 hours were 17.79 μg/mL and 12.17 μg/mL respectively.Apoptotic cells were observed and flow cytonetry analy-sis in dicated that the apoptosis rate of cells treated with different concentrations of β-Carboline alka-loids(0,5,10,20,40 μg/mL)for 24 and 48 hours were 1.66%,11.27%,20.32%,30.66%, 41.42%;3.84%,15.29%,23.34%,34.87%,49.54%,respectively.Increasing in apoptosis rate of SGC-7901 cells was associated with drug concentration.Typical DNA Ladder was detected in DNA agarose electrophoresis.Conclusion β-Carboline alkaloids can inhibit the proliferation and in-duce the apoptosis of SGC-7901 cells.
