Cloning of canine GM-CSF and SCF genes.
- Author:
Il Seob SHIN
1
;
Myung Jin NAM
;
Seong Jun PARK
;
Hwa Young YOUN
;
Hong Ryul HAN
Author Information
1. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Cats;
Cattle;
*Cloning, Molecular;
Codon;
DNA, Complementary/analysis/*genetics;
Dogs/blood/*genetics;
Gametogenesis;
Gene Amplification;
Granulocyte-Macrophage Colony-Stimulating Factor/chemistry/*genetics;
Hematopoiesis;
Humans;
Mice;
Molecular Sequence Data;
Open Reading Frames;
Reverse Transcriptase Polymerase Chain Reaction/methods;
Sequence Alignment;
Sequence Homology, Amino Acid;
Sheep;
Stem Cell Factor/chemistry/*genetics;
Swine
- From:Journal of Veterinary Science
2001;2(3):159-166
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cytokines have pleiotropic regulatory effects on hematopoietic cells and many other cell types that participate in host defence and repair processes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages and regulates the biological functions expressed by mature cells of these lineages. Stem cell factor (SCF) is a multifunctional cytokine involved in hematopoiesis, melanogenesis and gametogenesis. In order to determine the complementary DNA (cDNA) of canine GM-CSF and canine SCF, cDNA clones were generated from lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMCs) and bone marrow cells by reverse transcription PCR amplification. The canine GM-CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53-75% homology with those of human, cat, sheep, pig, cow and mouse, Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81-92% homology with those of human, cat, pig, cow and mouse.
