The clinical significance of detecting MMP-2mRNA and MMP-14mRNA with peritoneal lavage fluid in patients with gastric cancer
10.7619/jcmp.201409017
- VernacularTitle:胃癌患者术中腹腔冲洗液 MMP-2mRNA、MMP-14 mRNA 检测的临床意义
- Author:
Yibo WANG
1
;
Yulin TAN
;
Yawei SUN
;
Liming TANG
Author Information
1. 江苏大学附属武进医院 普外科
- Keywords:
gastric carcinoma;
matrix metalloproteinase-2;
matrix metalloproteinase-14;
peritoneal lavage fluid;
peritoneal metastasis
- From:
Journal of Clinical Medicine in Practice
2014;(9):61-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of detecting MMP-2mRNA and MMP-14mRNA with peritoneal lavage fluid in patients with gastric cancer.Methods The ex-pression of 98 patients of MMP-2mRNA and MMP-14 mRNA in peritoneal lavage fluid were de-teced by RT-PCR in patients with gastric cancer.30 patients with benign peritoneal lavage fluid were in the negative control group.Results The positive expression rates of MMP-2mRNA and MMP-14mRNA in the peritoneal lavage fluid in patients with gastric cancer were 42 /98(42.9%) and 40 /98(40.8%).but they were 2 /30(6.67%)、3.33% (1 /30)respectively in normal control (P <0.01).The positive expression rate of MMP-2mRNA and MMP-14mRNA were related to the depth of invasion,peritoneal metastasis,macroscopic type (P <0.01).Conclusion RT-PCR de-tection on the expression of MMP-2mRNA and MMP-14mRNA in peritoneal lavage fluid in patients with gastric cancer can be used to predict the peritoneal metastases.MMP-2 and MMP-14 may be involved in the peritoneal metastasis process.
