An experimental study on transplantation of granular fat combined micronized acellular dermal matrix
10.3760/cma.j.cn114453-20220923-00292
- VernacularTitle:颗粒脂肪与脱细胞真皮基质微粒联合移植的实验研究
- Author:
Mingmin YE
1
;
Guangtong CAO
;
Yi LIU
Author Information
1. 解放军联勤保障部队第九四○医院烧伤整形科，兰州 730050
- Keywords:
Adipose tissue;
Transplantation;
Micronized dermal acellular matrix;
Soft tissue filling;
Injection beauty
- From:
Chinese Journal of Plastic Surgery
2023;39(11):1236-1243
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether the combined injection of micronized acellular dermal matrix(mADM) and granular fat can improve the volume retention rate of the graft and the optimal mixture ratio of these two elements.Methods:From November 2022 to March 2023, the inguinal fat of rats was collected from the Animal Experiment Department of the 940th Hospital of the Joint Logistics Support Force of PLA, and then mixed with mADM in different proportions. Subsequently, different mixtures were injected into both sides of the back in another group of 15 rats: granular fat group (control group, C1), mADM group(control group, C2), granular fat+ mADM(1∶1)group(experimental group, E1), granular fat+ mADM(2∶1) group (experimental group, E2), granular fat+ mADM(4∶1) group(experimental group, E3), granular fat + mADM(6∶1) group (experimental group, E4), and 0.5 ml was injected in each group. At the 4th week, 8th week and 12th week, 5 specimens were randomly selected to detect the volume of grafts, histological analysis and neovascularization of grafts. SPSS 25.0 software was used for statistical analysis. Repeated measures analysis of variance was used to compare the data at multiple time points among multiple groups, and one-way analysis of variance was used to compare the data at each time point among multiple groups. P<0.05 was considered statistically significant. Results:The volume of the grafts in the 6 groups changed significantly with time, and the difference was statistically significant ( P <0.05). At the 4th, 8th and 12th week after injection, the volume of C2 group was larger than that of C1 group. The histological analysis showed that there was no obvious inflammatory cell infiltration in the early stage of C2 group, and the inflammatory reaction gradually occurred after 8 weeks. In the C1 group, vacuolization was observed, and the inflammatory cell infiltration was obvious. The larger the proportion of mADM and granular fat mixed injection, the more collagen fibers arranged regularly. There was a significant difference in the number of neovascularization among the 6 groups at the 4th week after transplantation ( P<0.05), but there was no significant difference at the 8th and 12th week ( P>0.05). Conclusion:mADM combined with granular fat injection can effectively maintain the volume of injection for a long time, and more suitable effect is achieved when the two are mixed at a ratio of 1∶1.
