Expression of COX-2 and IDO by Uteroglobin Transduction in NSCLC Cell Lines.
10.4046/trd.2009.66.4.274
- Author:
Gun Min PARK
1
;
Sang Min LEE
;
Jae Joon YIM
;
Seok Chul YANG
;
Chul Gyu YOO
;
Choon Taek LEE
;
Sung Koo HAN
;
Young Soo SHIM
;
Young Whan KIM
Author Information
1. Department of Internal Medicine, Dongguk University Ilsan Hospital, Dongguk University College of Medicine, Gyeongju, Korea.
- Publication Type:Original Article
- Keywords:
Uteroglobin;
Cyclooxygenase 2;
Indoleamine 2;
3-dioxygenase;
Interferon-gamma;
Immune tolerance
- MeSH:
Carcinoma, Non-Small-Cell Lung;
Cell Line;
Constitution and Bylaws;
Cyclooxygenase 2;
Immune Tolerance;
Indoleamine-Pyrrole 2,3,-Dioxygenase;
Interferon-gamma;
Kynurenine;
Lung;
Prostaglandin-Endoperoxide Synthases;
RNA, Small Interfering;
Tryptophan;
Uteroglobin
- From:Tuberculosis and Respiratory Diseases
2009;66(4):274-279
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Uteroglobin (UG) is a secretary protein that has strong immunomodulatory properties, and which is synthesized in most epithelia including lung tissue. Overexpression of UG is associated with decreased expression of cyclooxygenase (COX)-2 and suppression of cancer cell growth. Indoleamine 2,3-dioxygenase (IDO) catalyzes tryptophan along the kynurenine pathway, and both the reduction in local tryptophan and the production of tryptophan metabolites contribute to the immunosuppressive effects of IDO. METHODS: In this study, we investigated the pattern of expression of COX-2 and IDO, and the effect of UG transduction in the expression of COX-2 and IDO in several non-small cell lung cancer cell lines, especially A549. RESULTS: Both COX-2 and IDO were constitutionally expressed in A549 and H460 cells, and was reduced by UG transduction. In A549 cells, the slightly increased expression of COX-2 and IDO with the instillation of interferon-gamma (IFN-gamma) was reduced by UG transduction. However, the reduced expression of COX-2 and IDO by UG transduction was not increased with IFN-gamma instillation in A549 cells. In both the A549 COX-2 sense and the A549 COX-2 anti-sense small interfering RNA (siRNA)-transfected cells, IDO was expressed; expression was reduced by UG transduction, irrespective of the expression of COX-2. CONCLUSION: The results suggest that the anti-proliferative function of UG may be associated with the immune tolerance pathway of IDO, which is independent of the COX-2 pathway.
