Effect of silencing syndecan-1 on migration, invasion and angiogenesis of venous endothelial cells
10.3760/cma.j.cn114453-20210731-00326
- VernacularTitle:沉默多配体蛋白聚糖-1对静脉内皮细胞迁移、侵袭及血管生成能力的影响
- Author:
Dexin ZHANG
1
;
Junjie LIN
;
Junbo QIAO
;
Junhua LI
;
Haoran ZHANG
;
Wenqiu WANG
;
Yujiao WANG
Author Information
1. 郑州大学第三附属医院血管瘤外科河南省血管瘤与血管畸形医学重点实验室，郑州 450003
- Keywords:
Vascular malformations;
Syndecan-1;
Human umbilical vein endothelial cells (HUVECs);
Migration;
Invasion;
Angiogenesis
- From:
Chinese Journal of Plastic Surgery
2022;38(5):550-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of syndecan-1 (SDC1) in human venous malformations and the effect of syndecan-1 (SDC1) silences on migration, invasion and angiogenesis of human umbilical vein endothelial cells (HUVECs).Methods:Pathological samples of venous malformation were selected from 20 patients with hemangioma surgically resected in the Third Affiliated Hospital of Zhengzhou University from December 2020 to June 2021 (10 males and 10 females, aged 1-57 years, median age 7 years ). HUVECs was purchased from American Type Culture Collection (ATCC ). Immunohistochemistry was used to detect the expression of SDC1 in venous malformations. The cytological experiments of HUVECs cultured in vitro were divided into two groups, transfected SDC1 small interfering RNA (siRNA) (si-SDC1 group) and control siRNA (control group), respectively. The siRNA with the best silencing effect was selected by real-time quantitative PCR and Western Blot method for follow-up experiments. Then the ability of HUVECs migration, invasion and angiogenesis was detected by scratch test, Transwell migration and invasion test and angiogenesis test, respectively.Results:SDC1 was positive in 13 of the 20 venous malformations and was mainly expressed in the cytoplasm of venous endothelial cells. Compared with HUVECs cells in NC group, the expression of SDC1 mRNA and protein in HUVECs cells in si-SDC1 group decreased, indicated that the synthesized siRNA targeting SDC1 could silence the expression of SDC1 , and the ability of HUVECs migration, invasion and angiogenesis were enhanced in si-SDC1 group.Conclusions:SDC1 was positively expressed in venous malformations. After silencing SDC1, the migration, invasion and tube formation ability of HUVECs were enhanced.
