NF-kappa B activation following optic nerve transection.
- Author:
Jun Sub CHOI
1
;
Sungjoo KIM-YOON
;
Choun Ki JOO
Author Information
- Publication Type:Original Article
- MeSH: Animal; Apoptosis/physiology; Axotomy; Immunohistochemistry; Male; Microscopy, Electron; NF-kappa B/biosynthesis*; Optic Nerve/surgery; Rats; Rats, Sprague-Dawley; Retinal Ganglion Cells/ultrastructure; Retinal Ganglion Cells/pathology*; Retinal Ganglion Cells/metabolism; Substances: NF-kappa B
- From:Korean Journal of Ophthalmology 1998;12(1):19-24
- CountryRepublic of Korea
- Language:English
- Abstract: In order to elucidate in vivo neuronal cell death in the retina, and involvement of NF-kappa B in this process, we studied the degeneration of retinal ganglion cells (RGCs) and the activation of NF-kappa B after transection of the optic nerve of adult rat at 5 mm from the eyeball. The morphology of dying ganglion cells in the retinal ganglion cell layer was observed by light and electron microscopy, the activation of NF-kappa B was investigated immunohistochemically. Seven and 14 days post-axotomy, dying cells contained pyknotic nuclei. The death of retinal ganglion cells involved apoptosis, activation of NF-kappa B (p50 and p65) was prominent in a time dependent manner. We observed axotomy-induced NF-kappa B activation, which may mediate apoptosis of retinal ganglion cells.
