An in vitro three-dimension model for angiogenesis of Kaposiform hemangioendothelioma
10.3760/cma.j.cn114453-20200622-00378
- VernacularTitle:卡波西样血管内皮瘤体外血管生成三维模型的建立
- Author:
Zhengwei SUN
1
;
Changxian DONG
Author Information
1. 广东省妇幼保健院放射科，广州 511422
- Keywords:
Endothelial cells;
In vitro techniques;
Kaposi-form hemangioendothelioma
- From:
Chinese Journal of Plastic Surgery
2021;37(8):894-898
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and feasibility of an in vitro three-dimensional model for angiogenesis of Kaposiform hemangioendothelioma (KHE). Methods:In April 2019, a 4-month-old boy with KHE received surgery in the department of hemangioma of Henan Provincial People’s Hospital. The tumor tissue was used for the angiogenesis model. Treated tissue was planted in a fibrin gel culture system constructed by a double-layer sandwich method to establish a three-dimensional angiogenesis model. Optimum humidity and constant temperature was provided for the culture system, and the medium was changed every other day. The growth of model was observed every day. When the model became stable, well-grown models were made into frozen sections for HE and immunohistochemistry staining.Results:The model formed newborn vascular buds within 2-4 days and became stable in 12-16 days. The newborn vessels staggered into networks and showed obvious lumen-like structure. Apoptosis started after 26-30 days in most models, while no apoptosis appeared until 54 days in some ones. Morphological observation, HE staining, and immunohistochemistry examination confirmed that KHE endothelia have formed the new vessels.Conclusions:The culture system of fibrin gel is applicable for three-dimensional angiogenesis model of Kaposiform hemangioendothelioma. It is characterized with short culture time, long survival time, simple operation, low cost, and easy intervention.
