Expression and significance of the proteins in TSP-1 and NF-κB signal pathways of infantile capillary hemangioma
10.3760/cma.j.issn.1009-4598.2016.06.011
- VernacularTitle:TSP-1与NF-κB通路蛋白在婴幼儿毛细血管瘤中的表达
- Author:
Yongting ZHANG
1
;
Weili XU
;
Suolin LI
;
Zhiyong ZHONG
;
Yingchao LI
;
Wenbo WANG
;
Chi SUN
Author Information
1. 河北医科大学第二医院小儿外科
- Keywords:
Thrombospondin-1;
Nuclear factor kappa B;
Hemangioma,capillary;
Pathogenesis
- From:
Chinese Journal of Plastic Surgery
2016;32(6):441-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the significance of the relative proteins in thrombin sensitive protein(TSP-1) and nuclear factor kappa B (NF-κB) signal pathways by detecting their expressions in different phases of hemangioma and the relations between them and microvessel density(MVD).Methods Forty cases with hemangioma were randomly selected and divided into proliferative group(n =21) and involutional group(n =19) according to Mulliken standard.The expressions of TSP-1,CD36,p59fyn,Caspase-3,p38MAPK,NF-κBp65,p-IκBα,p-IKKβ,VEGF and CD34 in endothelial cells were detected by immunohistochemistry.The relevance between the proteins in TSP-1 and NF-κB signal pathways and MVD were compared and analyzed respectively.Results The expressions of TSP-1,CD36,p59fyn,Caspase-3,p38MAPK in proliferative hemangioma were all lower than those in involutional hemangioma (F=32.582,47.575,11.645,18.824,13.140;P =0.000,0.000,0.002,0.000,0.001).Conversely,the expressions of NF-κBp65,p-IκBα,p-IKKβ,VEGF in proliferative hemangioma were higher than those in involutional hemangioma(F =7.807,6.323,5.988,4.216;P =0.009,0.018,0.021,0.049).Meanwhile,MVD were different between the two groups(F =7.256,P =0.012).The expressions of TSP-1,CD36,p59fyn and MVD showed a negative relationship(rs =-0.420,-0.519,-0.388;P =0.021,0.003,0.034).Conversely,the expressions of NF-κBp65,pvIκBα,VEGF and MVD showed a positive relationship(rs =0.666,0.611,0.515;P =0.000,0.000,0.004).The expression of TSP-1 and NF-κBp65 showed a negative relationship (rs =-0.413,P =0.023).Conclusions TSP-1 signal pathway and NF-κBp65 signal pathway may be interactive in evolution of infantile hemangioma,which has a potential role by effecting the progress of angiogenesis.
