Differential expression profile of long non-coding RNA in regressive scar and mature scar
10.3760/cma.j.issn.1009-4598.2016.04.013
- VernacularTitle:消退期瘢痕与成熟期瘢痕组织中lncRNA表达谱的差异分析
- Author:
Jun LI
1
;
Qian LI
;
Yanli GAO
;
Bei ZHOU
;
Jingyun LI
Author Information
1. 210004,南京医科大学附属南京妇幼保健院医疗美容科
- Keywords:
RNA;
Oligonucleotide array sequence analysis;
Cicatrix
- From:
Chinese Journal of Plastic Surgery
2016;32(4):298-302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression profile of long non-coding RNA (lncRNA) in regressive scar and mature scar.Methods Hematoxylin-eosin staining and Masson staining were used to examine the histology of regressive scar and mature scar.The technique of IncRNA microarray was used to test the IncRNA expression profile in regressive scar and mature scar.The raw data of lncRNA were pretreated for normalization.Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was further performed.Four lncRNA expressions were validated by quantitative PCR.Results Compared with mature scar,more fibroblasts and deeper Masson staining color were seen in the regressive scar,a total of 1 275 upregulated more than 3-fold were found in the regressive scar.And 596 downregulated more than seventy percent lncRNA were found in the regressive scar.Pathway analysis indicated that chemokine signaling pathway,TNF signaling pathway,insulin secretion and focal adhesion would play important roles in scar development.Conclusions lncRNA expression profiles in the regressive scar is markedly different in comparison with the mature scar,revealing that lncRNA may participate in pathophysiological process of scar maturation.
