Mechanism of TRIM27 promoting inflammatory response in lung cancer cells
10.3760/cma.j.cn112152-20191204-00784
- VernacularTitle:三结构域蛋白27在非小细胞肺癌炎症反应发生中的机制
- Author:
Enjiu CHEN
1
;
Sheng CHEN
;
Fanglian ZHOU
Author Information
1. 温州医科大学附属平阳医院呼吸内科 325400
- Keywords:
Lung neoplasms;
Tripartite motif-containing 27;
Inflammatory response;
Mechanism
- From:
Chinese Journal of Oncology
2021;43(10):1076-1081
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of tripartite motif-containing 27 (TRIM27) expression promoting inflammatory response in non-small lung cancer cells.Methods:Ten cases of lung cancer tissues and their matched normal tissue (the distance was 5 cm of the tumor marginal) from patients underwent resection in the People′s Hospital of Pingyang Hospital Affiliated to Wenzhou Medical University were collected. The expression of TRIM27 was identified by using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot. TRIM27 knockdown experiment included negative control (NC TRIM27) group, TRIM27 short interfering RNA (siRNA) group, NC TRIM27+ TNF-α group and TRIM27 siRNA+ TNF-α group. Interlukin-6 (IL-6) knockdown experiment included NC IL-6 group and IL-6 siRNA group. The protein expressions of TRIM27, TNFR1, TNFR2 and some TNFR related inflammation factors were verified by qRT-PCR and WB. Results:The expression levels of TRIM27 in NSCLC tissues of different stages (stage Ⅰ: 2.81±0.58, stage Ⅱ: 3.32±1.38, stage Ⅲ: 3.67±1.24) was higher than that in the adjacent normal tissues (1.01±0.15, 0.92±0.10 and 1.05±0.12, P<0.05). The expression levels of TRIM27 mRNA in NC TRIM27 group and NC TRIM27+ TNF-α group were 0.94±0.12 and 1.67±0.03, and the expression levels of TRIM27 protein were 0.31±0.02 and 0.38±0.01, respectively ( P<0.05). The expression levels of IL-6 mRNA in NC TRIM27+ TNF-α group and TRIM27 siRNA+ TNF-α group were 11.35±0.12 and 5.62±0.15, respectively, and the expression levels of VCAM-1 mRNA were 18.75±0.17 and 9.35±0.11, respectively. STAT3 mRNA expression levels were 16.54±0.10 and 8.12±0.10, respectively, with statistical significance ( P<0.05). The expression levels of IL-6 mRNA in NC IL-6 group and IL-6 siRNA group were 1.10±0.07 and 0.52±0.16, respectively, and the expression levels of STAT3 mRNA were 1.01±0.01 and 0.48±0.12, respectively. The expression levels of TRIM27 mRNA were 1.03±0.01 and 0.30±0.11, respectively, with statistical significance ( P<0.05). Conclusion:The upregulation of TRIM27 in NSCLC tissue and cells promotes the expression of TNF-α, and may activate inflammatory response by regulating TNF-α-induced IL-6/STAT3 signaling pathway.
