miR-758-3p inhibits the proliferation and invasion of non-small cell lung cancer cells by targeting NUSAP1
10.3760/cma.j.cn112152-20190727-00472
- VernacularTitle:miR-758-3p通过靶向核仁纺锤体相关蛋白1抑制非小细胞肺癌细胞增殖和侵袭
- Author:
Xiaomin LI
1
;
Zhe YU
;
Mei HUAI
;
Hongyong HAN
;
Shanshan CAO
Author Information
1. 华北石油管理局总医院病理科，河北省任丘市 062552
- Keywords:
Carcinoma, non-small cell lung;
Micro-758-3p;
NUSAP1;
Proliferation;
Migration;
Invasion
- From:
Chinese Journal of Oncology
2021;43(1):113-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the biological function of miR-758-3p and the underlying mechanism in non-small cell lung cancer (NSCLC).Methods:miR-758-3p mimics was transfected to A549 NSCLC cells, miRNA control was used as a negative control, cells transfected with nucleolar and spindle associated protein 1 (NUSAP1)-overexpression vector indicated as NUSAP1 group, cells co-transfected with miR-758-3p mimics and NUSAP1-overexpression vector indicated as miR-758-3p mimics+ NUSAP1 group. The effects of miR-758-3p on the proliferation, migration and invasion abilities of A549 cells were detected by cell counting kit-8 (CCK-8) and Transwell assays, respectively. Bioinformatics and dual luciferase reporter assay were used to predict and verify the target gene of miR-758-3p.Results:The expressions of miR-758-3p and NUSAP1 in A549 cells were significantly up-regulated at 24 hours after transfection with miR-758-3p mimics ( P<0.05). Compared with the miRNA control group (1.15±0.06), the OD value of miR-758-3p mimic group (0.78±0.06) was significantly decreased at 72 hours after transfection ( P<0.05). The number of migrated cells of miR-758-3p mimic group (119.04±11.49) was significantly lower than that of the control group (271.38±19.05) ( P<0.05). The number of invaded cells of miR-758-3p mimic group (71.33±5.36) was significantly lower than the control group (164.30±8.11) ( P<0.05). The result of dual-luciferase reporter assay showed that NUSAP1 was a direct target of miR-758-3p. Moreover, up-regulation of NUSAP1 abolished the inhibitory effects of miR-758-3p on the proliferation, migration and invasion abilities of A549 cells. Conclusion:miR-758-3p inhibits the proliferation, migration and invasion of NSCLC cells by targeting NUSAP1.
