NUSAP1 promotes lung cancer progression by activating AKT/mTOR signaling pathway
10.3760/cma.j.cn112152-20200226-00132
- VernacularTitle:核仁纺锤体相关蛋白1激活AKT/mTOR信号通路促进肺癌进展
- Author:
Zhe YU
1
;
Xiaomin LI
;
Mei HUAI
;
Shanshan CAO
;
Hongyong HAN
;
Yanhua BI
Author Information
1. 华北石油管理局总医院心胸科，河北省任丘市 062552
- Keywords:
Lung neoplasms;
Nucleolar and spindle associated protein 1;
Cell proliferation;
Cell apoptosis
- From:
Chinese Journal of Oncology
2020;42(7):551-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effects of nucleolar and spindle associated protein 1 (NUSAP1) on lung cancer and the related mechanisms.Methods:A549 cells were transfected with NUSAP1 siRNA, the cell proliferation, migration and invasion, and apoptosis were detected by CCK8, Transwell and flow cytometry, respectively. Western blot was used to detect the expressions of apoptosis and AKT/mTOR signal pathway related proteins.Results:Compared with the negative control group, the proliferation [(0.610±0.058) vs (1.724±0.067), P<0.05], migration [(178.267±14.780) vs (272.464±36.232), P<0.05] and invasion [(73.527±6.617) vs (120.585±13.235), P<0.05] of NUSAP1 deleted A549 cells were significantly inhibited, while the apoptosis [(3.572±0.214)% vs (11.358±1.047)%, P<0.05] was significantly increased. The expressions of apoptosis related protein Bax and active-caspase 3 were increased ( P<0.05), while the expressions of anti-apoptosis protein Bcl-2 and proliferation related protein P70, the phosphorylation levels of AKT and mTOR were reduced in NUSAP1 knockdown cells ( P<0.05). Conclusion:NUSAP1 knockdown can inhibit the proliferation, migration and invasion, and promote the apoptosis of tumor cells through suppressing AKT/mTOR signaling pathway in lung cancer cells.
