The Change of Detrusor Collagen according to the Detrusor Contractility in Benign Prostatic Hyperplasia.
- Author:
Jong Yun KIM
1
;
Seong Ho LEE
;
Ha young KIM
Author Information
1. Department of Urology, College of Medicine, Hallym University, Chuncheon, Korea.
- Publication Type:Original Article
- Keywords:
Prostatic hyperplasia;
Bladder;
Muscle contracion;
Collagen
- MeSH:
Blotting, Western;
Collagen Type I;
Collagen Type III;
Collagen*;
Humans;
Hydroxyproline;
Prostate;
Prostatic Hyperplasia*;
Urinary Bladder
- From:Korean Journal of Urology
2004;45(6):535-542
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aim of this study was to investigate the change of collagen content and the subtype of collagen in compensated and decompensated detrusors by benign prostatic hyperplasia (BPH). MATERIALS AND METHODS: A total of 62 patients with BPH who underwent transurethral resection of the prostate (TUR-P) was involved in this study. Preoperatively, the Watts factor (WF) was measured for detrusor contractile strength, and the patients were divided into 3 groups according to these measurements: group 1 consisted of patients with decreased contractile strength, group 2 consisted of patients with normal contractile strength, and group 3 consisted of patients with increased contractile strength. The chips of the detrusor were obtained at the time of TUR-P. Total collagen concentration was quantified by hydroxyproline assay. Immunohistochemical staining was performed for the expression and localization of type I and III collagen, and Western blot analysis was performed for the extent of expression of type I and III collagen. RESULTS: The total collagen concentrations of groups 1, 2, and 3, which significantly varied among the three groups, were 132.5+/-59.2, 265.1+/-96.8, and 437.6+/-162.5mug/mg, respectively (ANOVA test, p<0.05). In immunohistochemical staining and Western blot analysis, type I collagen was expressed similarly among the three groups. The expression of type III collagen was weakest in group 1 and strongest in group 3. There was a positive correlationship between WF and the expression of type III collagen in the detrusor layer. CONCLUSIONS: These data suggest that the change of total content and subtype of collagen in the detrusor muscle is the major histological change in BPH and type III collagen, which play an important role in detrusor contractility.
