Multiregion sequencing reveals intratumor heterogeneity in esophageal squamous cell carcinoma
10.3760/cma.j.issn.0253-3766.2016.09.005
- VernacularTitle:食管鳞状细胞癌癌内异质性的初步研究
- Author:
Wei CAO
1
;
Wei WU
;
Ming YAN
;
Fu TIAN
;
Hansong LIU
;
Jinwu WANG
;
Quanwu ZHANG
;
Yujie LI
;
Min LI
Author Information
1. 450007,郑州大学附属郑州中心医院转化医学中心
- Keywords:
Esophageal neoplasms;
Neoplasms,squamous cell;
Whole exome sequencing;
Heterogeneity,intratumor
- From:
Chinese Journal of Oncology
2016;38(9):660-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the intratumor heterogeneity of esophageal squamous cell carcinoma ( ESCC) . Methods We used whole?exome sequencing and array?based comparative genomic hybridization to profile mutations and changes in copy number from 11 regions within 2 cases of ESCC and from the metastatic lymph nodes. Results The numbers of somatic single nuclear polymorphisms ( SNPs ) in 4 regions within the tumors in case 1 and case 2 were 93±17 and 124±28, respectively. The majority of SNPs were non synonymous mutations, synonymous mutations, nonsense mutations and splicing junction mutations. The average indels in the 4 tumor regions of case 1 and case 2 were 40 ± 6 and 51 ± 3, respectively. These small indels mainly occurred in exonic ( frame?shift and non?frame?shift ) , untranslational regions of genes and splicing junction regions. All regions from a tumor exhibitedo bvious heterogeneity, and mutational similarity of all four regions within a tumor was less than 25%. Furthermore, gene copy number alteration ( gain or loss) varied among multiple regions of a tumor, and the similarity of gene copy number was less than 20%. Phylogenetic analysis of the somatic mutation frequency suggests that multiple, genomic heterogeneous clones co?exist within a primary ESCC, and metastatic subclones may evolve from the primary non?metastatic parental clone. These results indicated that a single?region sampling can not reflect the architecture of the genomic landscape of mutations in ESCC tumors. Conclusions Sequence analysis of whole genome exon in multiple regions can provide strong evidence for genomic heterogeneity in esophageal squamous cell carcinoma.
