Development of a Rat Model of Graded Contusive Spinal Cord Injury Using a Pneumatic Impact Device.
10.3346/jkms.2004.19.4.574
- Author:
Sang Jun YEO
1
;
Sung Nam HWANG
;
Seung Won PARK
;
Young Baeg KIM
;
Byung Kook MIN
;
Jeong Taik KWON
;
Jong Sik SUK
Author Information
1. Department of Neurosurgery, College of Medicine, Chung-Ang University, Seoul, Korea. tarheelk@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Models, Animal;
Spinal Cord Injuries;
Equipment and Supplies;
Equipment Design
- MeSH:
Animals;
Behavior, Animal/physiology;
Humans;
Laminectomy;
Male;
*Models, Animal;
Motor Activity/physiology;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Recovery of Function;
Research Support, Non-U.S. Gov't;
*Spinal Cord Injuries/pathology/therapy
- From:Journal of Korean Medical Science
2004;19(4):574-580
- CountryRepublic of Korea
- Language:English
-
Abstract:
An animal model of spinal cord trauma is essential for understanding the injury mechanisms, cord regeneration, and to aid the development of new therapeutic modalities. This study focused on the development of a graded experimental contusion model for spinal cord injury (SCI) using a pneumatic impact device made in Korea. A contusive injury was made to the dorsal aspect of the cord. Three trauma groups were defined according to the impact velocity (IV). A control group (n=6), received laminectomy only. Group 1 (n=10), 2 (n=10), and 3 (n=10) had IVs of 1.5 m/sec, 2.0 m/sec, and 3.5 m/sec respectively. Functional assessments were made up to the 14th day after injury. The cord was removed at the 14th post-injury day and prepared for histopathologic examination. Significant behavioral and histopathological abnormalities were found in control and each trauma group. All trauma groups showed severe functional impairment immediately after injury but following different rates of functional recovery (Fig. 5). As the impact velocity and impulse increased, the depth of contusive lesion revealed to be profound the results show that the rat model reproduces spinal cord lesions consistently, has a distinctive value in assessing the effects of impact energy.
