Expression of microRNA-29b in colorectal cancer and its relationship with clinicopathological features
10.3760/cma.j.issn.0253-3766.2016.06.006
- VernacularTitle:miR-29b在结直肠癌组织中的表达及其临床意义
- Author:
Xiao MA
1
;
Wei YUAN
;
Wanyan TANG
;
Jie MA
Author Information
1. 100021,国家癌症中心 北京协和医学院中国医学科学院肿瘤医院分子肿瘤学国家重点实验室
- Keywords:
Colorectal neoplasms;
MiR-29b;
Neoplasms metastasis;
Neoplasms stages;
Prognosis
- From:
Chinese Journal of Oncology
2016;38(6):430-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to detect the expression level of miR?29b in colorectal cancer ( CRC ) tissues and to analyze its relationship with clinicopathological features and prognosis. Methods The expression of miR?29b was detected by real?time quantitative PCR in 202 colorectal cancer tissues and adjacent colorectal tissues. Statistical analysis of the results was conducted using SPSS 16.0 software. Results The expression of miR?29b in colorectal cancer tissues and adjacent colorectal tissues was -7.761±0.115 and -7.150±0.137, respectively, with a significant difference between the two groups ( P<0.01) . Comparing colorectal cancer tissues with lymph node metastasis with those without lymph node metastasis, the expression of miR?29b (-7.528±0.158 vs. -7.988±0.164) was significantly increased (P<0.05). In addition, the expression of miR?29b in CRC according to TNM stage was -8.096±0.157 in stage Ⅰ/Ⅱ,-7.592±0.165 in stage Ⅲ and -6.603±0.468 in stageⅣpatients, showing a gradual increase depending on clinical staging ( P<0. 05 ) . Univariate Cox regression analysis indicated that lymph node metastasis, differentiation degree and TNM stage were significantly related to the postoperative survival of colorectal cancer patients ( P<0.001 ) . Multivariate Cox regression analysis indicated that differentiation degree and TNM stage were independent factors impacting the survival of colorectal cancer patients ( P<0.001) . Conclusions The expression level of miR?29b is reduced in colorectal cancer tissues compared with that in the adjacent colorectal tissues. High expression level of miR?29b is associated with lymph node metastasis and TNM stage of CRC. miR?29b may be a potential marker indicating colorectal cancer metastasis and malignant progression.
