Mifepristone inhibites the migration of endometrial cancer cells through regulating H19 methylation
10.3760/cma.j.issn.0253-3766.2016.06.003
- VernacularTitle:米非司酮通过调控H19基因甲基化抑制子宫内膜癌细胞的迁移
- Author:
Zhenzhen LU
1
;
Lei YAN
;
Hui ZHANG
;
Mingjiang LI
;
Xiaohui ZHANG
;
Xingbo ZHAO
Author Information
1. 山东大学附属省立医院妇产科
- Keywords:
Endometrial neoplasms;
Methylation;
Mifepristone;
H19 gene
- From:
Chinese Journal of Oncology
2016;38(6):411-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of mifepristone on the migration of human endometrial carcinoma cells. Methods A human endometrial carcinoma cell line, Ishikawa cells, was cultured in vitro and treated with mifepristone at different concentrations. Wound healing assay was applied to detect the migration of Ishikawa cells. RT?PCR and methylation?specific PCR (MSP) were used to detect the levels of H19 mRNA and its DNA methylation. Western?blot was used to detect the expressions of HMGA2 and epithelial to mesenchymal transition ( EMT ) related proteins. Results When treated with different concentrations of mifepristone for 48 hours, the width of scratch of the the control group, the 5 mg/L and the 10 mg/L mifepristone treatment groups were (4.18±0.07)mm,(4.68±0.07)mm, and(4.99±0.07) mm, respectively (P<0.05 for all) and treated with mifepristone for 72 hours, those were(3.46±0.07)mm, (4.29±0.07)mm, and(4.78±0.04)mm, respectively (P<0.05 for all). In the Ishikawa cells, mifepristone suppressed the transcriptional level of H19 through enhancing its promoter methylation, which resulted in inhibited expressions of HMGA2 and vimentin and increased expression of E?cadherin in a time? and concentration?dependent manner. Conclusion Mifepristone inhibits the migration of endometrial carcinoma cells partially through methylation?induced of transcriptional inhibition of H19, which results in the down?regulation of HMGA2 and vimentin and upregulation of E?cadherin.
