Associations between interleukin-17A expression and epithelial-mesenchymal transition in patients with hepatocellular carcinoma
10.3760/cma.j.issn.0253-3766.2015.08.005
- VernacularTitle:白细胞介素17 A与原发性肝细胞癌上皮间质转化的相关性
- Author:
Jun XU
1
;
Jian WANG
;
Yong HU
;
Lanlan CHENG
;
Hongyu YU
Author Information
1. 解放军第一0五医院病理科
- Keywords:
hepatocellular neoplasms;
Interleukin-17A;
Epithelial mesenchymal transition;
Prognosis
- From:
Chinese Journal of Oncology
2015;(8):585-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of interleukin?17A ( IL?17A ) in hepatocellular carcinoma ( HCCs ) tissues, and to analyze its relationship with clinicopathological characteristics and epithelial?mesenchymal transition ( EMT ) . Methods The expression of IL?17A, E?cadherin, vimentin proteins and Snail mRNA were detected by immunohistochemistry and in situ hybridization histochemistry in the hepatocellular carcinoma ( HCC) tissues of 74 patients. Results IL?17A staining was detected in 54.1%(40/74) specimens of human HCCs, but only 25.0%(5/20) in corresponding peritumoral tissues (P<0.05) . The positive rate of IL?17A expression in HCC patients with grade Ⅲ+Ⅳ and UICC stage Ⅲ+Ⅳtumors was significantly higher than those with gradeⅠ+Ⅱand UICC stageⅠ+Ⅱtumors. The expression of IL?17A was positively correlated with portal vein tumor thrombus and microvascular invasion(all P<0.05). The 1?and 2?year recurrence?free survival rates were 27.6% and 17.2% in the patients with positive IL?17A expression, but 79.3% and 58.5% in IL?17A?negative HCCs. The 1? and 2?year overall survival rates were 69.0% and 27.8% in the cases with positive IL?17A expression, while 91.3% and 87.0% in IL?17A?negative cases. Patients with IL?17A?positive HCCs showed significantly shorter recurrence?free and overall survival compared with the patients with IL?17A?negative HCCs(P<0.05). Multivariate analysis indicated that IL?17A expression was an independent factor for recurrence?free and overall survival of HCCs. IL?17A?positive HCCs were characterized by increased expression of vimentin(r=0.492, P<0.01) or Snail (r=0.410, P<0.05) and loss of E?cadherin expression(r=-0.404, P<0.05). Conclusions Our results suggest that IL?17A is closely related to epithelial?mesenchymal transition in hepatocellular carcinoma. IL?17A?positive hepatocellular carcinoma demonstrates more aggressive biological behavior, and IL?17A may serve as a potential prognostic marker for this cancer.
