Expression of osteopontin splice variant and its clinical significance in gastric cancer
10.3760/cma.j.issn.0253-3766.2015.06.006
- VernacularTitle:骨桥蛋白基因剪切体在胃癌组织中的表达及意义
- Author:
Xianjun SUN
1
;
Longgang WANG
;
Wenhong HOU
;
Yanliang LI
;
Liqing LIU
;
Wenshu ZUO
;
Jinming YU
Author Information
1. 山东省肿瘤医院胃肠外科
- Keywords:
Stomach neoplasms;
Osteopontin;
Gene expression;
Prognosis
- From:
Chinese Journal of Oncology
2015;(6):427-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of osteopontin ( OPN) splice variant mRNA, including the three isoforms OPN?A, OPN?B, and OPN?C, to explore its correlation with clinicopathologic features in gastric cancer, and to elucidate their role in tumor invasion and distant metastasis of gastric cancer. Methods The expression of OPN?A, OPN?B and OPN?C mRNA were detected by real?time reverse transcriptase?polymerase chain reaction in 66 gastric cancer tissues. The relationship between the expression of OPN?A, OPN?B and OPN?C mRNA and clinicopathologic features of gastric cancer was analyzed. Results The expression of OPN?C mRNA in the gastric cancer tissue was 3. 21?fold higher than that in peritumoral mucosal tissue, showing a significant difference between them (P<0.001). OPN?C mRNA expression was correlated with the depth of tumor invasion, tumor diameter, lymph node meatastasis, distant meatastasis and had no correlation with differentiation grades. The low expression of OPN?C mRNA was correlated with long survival benefit ( P=0.03) . The expression of OPN?A and OPN?B mRNA had no significant relationship with clinicopathologic features of gastric cancer. Conclusions One of the isoform of osteopontin ( OPN) OPN?C mRNA is overexpressed in gastric cancer. The overexpression of OPN?C mRNA may reflect the progression and is associated with the prognosis of gastric cancer. OPN?C mRNA may have value as a prognostic biomarker in gastric cancer. However, the expression of OPN?A and OPN?B are not correlated with the progression and metastasis of gastric cancer.
