OLC1 protein levels in plasma of patients with non-small cell lung cancer and its clinical application
10.3760/cma.j.issn.0253-3766.2014.05.009
- VernacularTitle:OLC1蛋白在非小细胞肺癌患者外周血血浆中的水平及其临床意义
- Author:
Longhai YANG
1
;
Ting XIAO
;
Jinjing TAN
;
Shiping CHEN
;
Yanning GAO
;
Shujun CHENG
;
Xiangyang LIU
;
Kelin SUN
Author Information
1. 100021,北京协和医学院 中国医学科学院肿瘤医院胸外科
- Keywords:
Carcinoma,non-small-cell lung;
OLC1;
Plasma;
Antibodies,monoclonal
- From:
Chinese Journal of Oncology
2014;(5):362-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to detect the plasma concentration of OLC 1 ( overexpressed in lung cancer 1 ) protein as a potential cancer biomarker , and evaluating its clinical application value in the diagnosis of non-small cell lung cancer (NSCLC).Methods We prepared OLC1 antibody with OLC1 full length protein, in 5-6-week old Bal B/c mice.Each mouse was immunized four times at a dose of 15-30μg antigen protein , and the interval between two consecutive immunizations was two weeks.Antibody screening was made by ELISA and Western blot , and a double antibody sandwich ELISA kit was developed .We used this established ELISA kit to detect the plasma concentration of OLC 1 protein in 281 NSCLC patients and 92 gender-and age-matched healthy controls .Area under the receiver operating characteristic curve (AUC) was used to evaluate the detection efficacy of OLC 1.Results We obtained 11 OLC1 monoclonal antibodies and successfully established the ELISA kit to detect the plasma concentration of OLC1 with a detection range from 1.95 ng/ml to 62.50 ng/ml.OLC1 concentration in the case group (124.69 ng/ml) was significantly higher than that in the control group (67.07 ng/ml, P<0.001).In the scenario of distinguishing NSCLC from control group , AUC result was 0.69.When the cut-off was set at 67.72 ng/ml, the sensitivity and specificity was 84.4%and 51.1%, respectively.In term of distinguishing early lung cancer (IA) from normal controls, the AUC, sensitivity and specificity were 0.68, 77.8% and 54.4%, respectively .Conclusion The plasma concentration of OLC 1 protein is significantly elevated in NSCLC patients.OLC1 may be as a potential cancer biomarker applied in clinical diagnosis .
