Study on the Fluorescent Body (F-body) of the Human Spermatozoa.
- Author:
Dong Chull SHIN
1
;
Moo Sang LEE
Author Information
1. Department of Urology, College of Medicine, Yonsei University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
human spermatozoa;
fluorescent body
- MeSH:
Adult;
Ethanol;
Humans*;
Male;
Quinacrine;
Semen Analysis;
Sperm Motility;
Spermatozoa*
- From:Korean Journal of Urology
1981;22(5):407-411
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study seminal fluid samples were obtained from 20 normal adult males to know the percentage of the spermatozoa containing the fluorescent body (F-body), after staining the seminal fluid with 0.5% aqueous quinacrine HCI. In this study the preparations were observed with an AO fluorescent microscope. The following results were obtained. 1) In 20 men, the mean percentage of F-body containing spermatozoa was 31.3%. 2) There was no relationship between sperm density in semen analysis and F-body rate 3) There was no relationship between sperm motility in semen analysis and F-body rate. 4) In 5 men, methyl alcohol-acetic acid (3:1) preparations, as a fixation solution, showed better F-body rate than that of ethyl alcohol preparations. 5) In 4 men, the preparations which were fixed and stained within 2 hours after obtaining seminal fluid showed better F-body rate than that fixed and stained after 6 hours. 6) In 10 men, after low speed centrifuging of the seminal fluid, the deposit preparations, showed decreased mean percentage of F-body containing spermatozoa by 4.2%.
