Expression and clinical significance of MOC-31 mRNA in pleural fluid of lung cancer patient
10.3321/j.issn:0253-3766.2008.01.012
- VernacularTitle:肺癌患者胸水中MOC-31 mRNA的表达及临床意义
- Author:
Ying SUN
1
;
Guang-Ping WU
;
Yu-Jie ZHAO
;
Shao-Cheng WANG
;
En-Hua WANG
Author Information
1. 中国医科大学附属第一医院
- Keywords:
Lung neoplasms;
Hydrothorax,pleural fluid:RT-PCR
- From:
Chinese Journal of Oncology
2008;30(1):48-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of MOC-31 mRNA and its clinical significance in pleural fluid of lung cancer patient.Methods Reverse transcriptase polymerase chain reaction (RT-PCR)was used to detect MOC-31 mRNA in pleural fluid from 74 lung cancer patients and 40 Datients with benign lung diseases,and the result was compared with conventional cytological diagnosis.Results The MOC-31 mRNA was detected in 91.9%(68/74)of the pleural fluid from lung cancer patients,and only in 10.O%(4/40)from the patients with benign lung diseases with a statistically significant difference between two groups(x2=74.83,P<0.01).The positive expression of MOC-31 mRNA in pleural fluid was not correlated with histo-pathological types of lung cancer(P>0.05).The diagnostic sensitivity and accuracv of MOC-3 1 mRNA detection were significantly higher than that of conventional cytological diagnosis in pleural fluid of lung cancer patients(P<0.01).Conclusion MOC-31 mRNA as a molecular marker may be helpful to detect pleural micrometastatic cancer cells in pleural fluid of lung cancer patient.
